Ground-beyond-Zero
Expression of exogenous protein in the egg white of transgenic chicken - Printable Version

+- Ground-beyond-Zero (https://www.ground-beyond-zero.com)
+-- Forum: General Discussion: Conspiracy-Ufo's-Paranormal,World change (/forumdisplay.php?fid=32)
+--- Forum: DOOMED- GZ 6/22/2011 (/forumdisplay.php?fid=33)
+--- Thread: Expression of exogenous protein in the egg white of transgenic chicken (/showthread.php?tid=83860)



Expression of exogenous protein in the egg white of transgenic chicken - programmer - 03-28-2020 01:59 AM

Using a replication-deficient retroviral vector based on the avian leukosis virus (ALV), we inserted into the chicken genome a transgene encoding a secreted protein, beta-lactamase, under the control of the ubiquitous cytomegalovirus (CMV) promoter. Biologically active beta-lactamase was secreted into the serum and egg white of four generations of transgenic chickens. The expression levels were similar in successive generations, and expression levels in the magnum of the oviduct were constant over at least 16 months in transgenic hens, indicating that the transgene was stable and not subject to silencing. These results support the potential of the hen as a bioreactor for the production of commercially valuable, biologically active proteins in egg white.


The use of chicken as an animal model has increased in recent decades due to its relatively short generation time, plentiful reproductive capacity, and suit- ability for use as a human disease model, including in studies of cancer and inherited disease [12e14]. Regarding the production of recombinant protein, a single egg white of chicken contains ~6.5 g of protein and is composed of 10 major proteins [15]. In addition, more than half of egg-white proteins are encoded by a single gene, ovalbumin (OV), which means that chicken bioreactor systems are more competitive for mass production and subsequent purification of exogenous proteins compared to systems based on other animals ...
...

Regarding the production of recombinant protein, a single egg white of chicken contains ~6.5 g of protein and is composed of 10 major proteins . In addition, more than half of egg-white proteins are encoded by a single gene, ovalbumin (OV), which means that chicken bioreactor systems are more competitive for mass production and subsequent purification of exogenous proteins compared to systems based on other animals. More importantly, genetic stability among generations is an advantage of the chicken system for the production of therapeutic antibodies . ...
... In addition, more than half of egg-white proteins are encoded by a single gene, ovalbumin (OV), which means that chicken bioreactor systems are more competitive for mass production and subsequent purification of exogenous proteins compared to systems based on other animals [15,16]. More importantly, genetic stability among generations is an advantage of the chicken system for the production of therapeutic antibodies ..

https://www.researchgate.net/publication/11441137_Expression_of_exogenous_protein_in_the_egg_white_of_transgenic_chicken


RE: Expression of exogenous protein in the egg white of transgenic chicken - IJD - 03-28-2020 01:59 AM

[Image: tumblr_m22uuvoac81r2kjx1.gif]


RE: Expression of exogenous protein in the egg white of transgenic chicken - sysop - 03-28-2020 02:03 AM

Chicken,and all with wings... no thank you....


coffee1